Some individuals experience immediate (Type I) allergy symptoms when exposed to pollens, dust, animal dander and foods. Previously, skin testing with its attendant patient’s risks and discomfort, was used to diagnose these IgE mediated allergy reactions (skin prick testing).
Recently, more convenient blood tests have been developed to detect IgE specific for many allergens. Diagnosing specific allergies is the first step in a program of patient health recovery, which can include immunotherapy, lifestyle management and permanent elimination of reactive foods.
Immuno’s Airborne & Food Allergy IgE detects serum IgE specific for airborne allergens and foods. The procedure involves incubation of serum with airborne and food allergenic extracts covalently bonded to a solid surface. Specific IgE (if present in serum) binds to fixed allergen.
Enzyme-labeled antiserum to human IgE is added and incubated. A photo-reagent substrate is added and an enzymatic reaction produces luminescence corresponding to the reactive antigen. The luminescence of each band is measured using a luminometer. The degree of transmittance is directly proportional to the allergic specific IgE. Antibody level is computer calculated and expressed in classification units, which correlate with RAST classes.